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Anti-RAB27A Antibody (3T522) 是一种抗体,靶向 RAB27A。Anti-RAB27A Antibody (3T522) 可用于 ELISA, FCM。
Anti-RAB27A Antibody (3T522) 是一种抗体,靶向 RAB27A。Anti-RAB27A Antibody (3T522) 可用于 ELISA, FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,300 | 5日内发货 | |
100 μL | ¥ 2,180 | 5日内发货 |
产品描述 | Anti-RAB27A Antibody (3T522) is an antibody targeting RAB27A. Anti-RAB27A Antibody (3T522) can be used in ELISA, FCM. |
别名 | Ras-related protein Rab-27A, Rab-27, RAB27, RAB 27A, GTP-binding protein Ram |
Ig Type | Rabbit IgG |
克隆号 | 3T522 |
交叉反应 | Human |
验证活性 | Overlay Peak curve showing PC3 cells stained with TMAH-01030 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
应用 | |
推荐剂量 | FCM:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. |
组织特异性 | Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human RAB27A |
抗原种属 | Human |
基因名称 | RAB27A |
基因ID | |
Uniprot ID | |
研究领域 | Signal transduction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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