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Anti-Pumilio 1 Antibody (3G125)
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产品编号 TMAC-03472
别名 PUML1, PUML 1, Pumilio1, pumilio homolog 1 (Drosophila), Pumilio homolog 1, PUMH1, PUMH 1, PUMH, PUM1_HUMAN, Pum1, PUM L1, PUM 1, KIAA0099, HsPUM
Anti-Pumilio 1 Antibody (3G125) 是一种 Rabbit 抗体，靶向 Pumilio 1。Anti-Pumilio 1 Antibody (3G125) 可用于 WB,ICC,IF,IHC,FCM,IP。
Anti-Pumilio 1 Antibody (3G125)
Anti-Pumilio 1 Antibody (3G125)
还可以产品编号 TMAC-03472 别名 PUML1, PUML 1, Pumilio1, pumilio homolog 1 (Drosophila), Pumilio homolog 1, PUMH1, PUMH 1, PUMH, PUM1_HUMAN, Pum1, PUM L1, PUM 1, KIAA0099, HsPUM
Anti-Pumilio 1 Antibody (3G125) 是一种 Rabbit 抗体，靶向 Pumilio 1。Anti-Pumilio 1 Antibody (3G125) 可用于 WB,ICC,IF,IHC,FCM,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-Pumilio 1 Antibody (3G125) is a Rabbit antibody targeting Pumilio 1. Anti-Pumilio 1 Antibody (3G125) can be used in WB,ICC,IF,IHC,FCM,IP. |
| 别名 | PUML1, PUML 1, Pumilio1, pumilio homolog 1 (Drosophila), Pumilio homolog 1, PUMH1, PUMH 1, PUMH, PUM1_HUMAN, Pum1, PUM L1, PUM 1, KIAA0099, HsPUM |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Western blot analysis of Pumilio 1 on SiHa cell using anti-Pumilio 1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. 6. ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Pumilio 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of 293T cells with Pumilio 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: human Pumilio 1 aa 1-200 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 126 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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