购物车
- 全部删除
您的购物车当前为空
Anti-PUMA Antibody (5V529)
还可以
产品编号 TMAC-03471
别名 PUMA/JFY1, PUMA alpha, PUMA, p53 Upregulated Modulator of Apoptosis, p53 up regulated modulator of apoptosis, JFY1, JFY 1, bcl-2 binding component 3, BCL2 binding component 3, BCL 2 binding component 3, BBC3_RAT, BBC3, BBC 3
Anti-PUMA Antibody (5V529) 是一种 Rabbit 抗体，靶向 PUMA。Anti-PUMA Antibody (5V529) 可用于 WB,ICC/IF,IHC,FCM。
Anti-PUMA Antibody (5V529)
Anti-PUMA Antibody (5V529)
还可以产品编号 TMAC-03471 别名 PUMA/JFY1, PUMA alpha, PUMA, p53 Upregulated Modulator of Apoptosis, p53 up regulated modulator of apoptosis, JFY1, JFY 1, bcl-2 binding component 3, BCL2 binding component 3, BCL 2 binding component 3, BBC3_RAT, BBC3, BBC 3
Anti-PUMA Antibody (5V529) 是一种 Rabbit 抗体，靶向 PUMA。Anti-PUMA Antibody (5V529) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。
联系我们获取更多批次信息
资源下载
产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PUMA Antibody (5V529) is a Rabbit antibody targeting PUMA. Anti-PUMA Antibody (5V529) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | PUMA/JFY1, PUMA alpha, PUMA, p53 Upregulated Modulator of Apoptosis, p53 up regulated modulator of apoptosis, JFY1, JFY 1, bcl-2 binding component 3, BCL2 binding component 3, BCL 2 binding component 3, BBC3_RAT, BBC3, BBC 3 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PUMA on different lysates using anti-PUMA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: K562. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human gastric carcimnoma tissue using anti-PUMA antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PUMA antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin. 6. ICC staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining PUMA in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with PUMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The expression of PUMA is regulated by the tumor suppressor p53. PUMA is involved in p53-dependent and -independent apoptosis induced by a variety of signals, and is regulated by transcription factors, not by post-translational modifications. After activation, PUMA interacts with antiapoptotic Bcl-2 family members, thus freeing Bax and/or Bak which are then able to signal apoptosis to the mitochondria. Following mitochondrial dysfunction, the caspase cascade is activated ultimately leading to cell death. Several studies have shown that PUMA function is affected or absent in cancer cells. Additionally, many human tumors contain p53 mutations, which results in no induction of PUMA, even after DNA damage induced through irradiation or chemotherapy drugs.Other cancers, which exhibit overexpression of antiapotptic Bcl-2 family proteins, counteract and overpower PUMA-induced apoptosis. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 18 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
SCI 文献
评论列表
预览
Related Tags: buy Anti-PUMA Antibody (5V529) | purchase Anti-PUMA Antibody (5V529) | Anti-PUMA Antibody (5V529) cost | order Anti-PUMA Antibody (5V529) | Anti-PUMA Antibody (5V529) molecular weight
嗨！有任何问题？点我咨询
评论内容