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Anti-PTPN14 Antibody (8D656) 是一种 Mouse 抗体,靶向 PTPN14。Anti-PTPN14 Antibody (8D656) 可用于 WB,ICC,IHC,FCM。
Anti-PTPN14 Antibody (8D656) 是一种 Mouse 抗体,靶向 PTPN14。Anti-PTPN14 Antibody (8D656) 可用于 WB,ICC,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,985 | 5日内发货 | |
100 μL | ¥ 2,980 | 5日内发货 |
产品描述 | Anti-PTPN14 Antibody (8D656) is a Mouse antibody targeting PTPN14. Anti-PTPN14 Antibody (8D656) can be used in WB,ICC,IHC,FCM. |
别名 | Tyrosine-protein phosphatase non-receptor type 14, PTPN14, PTPD2, Protein-tyrosine phosphatase pez, PEZ |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of PTPN14 on human PTPN14 ecombinant protein using anti-PTPN14 antibody at 1/1,000 dilution. 2. Western blot analysis of PTPN14 on HEK293 (1) and PTPN14-hIgGFc transfected HEK293 (2) cell lysate using anti-PTPN14 antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PTPN14 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PTPN14 antibody. Counter stained with hematoxylin. 5. ICC staining PTPN14 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with PTPN14 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 135 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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