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Anti-PSGL-1/CD162 Antibody (3U936) 是一种 Rabbit 抗体,靶向 PSGL-1/CD162。Anti-PSGL-1/CD162 Antibody (3U936) 可用于 FCM。
Anti-PSGL-1/CD162 Antibody (3U936) 是一种 Rabbit 抗体,靶向 PSGL-1/CD162。Anti-PSGL-1/CD162 Antibody (3U936) 可用于 FCM。
产品描述 | Anti-PSGL-1/CD162 Antibody (3U936) is a Rabbit antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (3U936) can be used in FCM. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 3U936 |
交叉反应 | Mouse |
验证活性 | Flow cytometric analysis of Mouse SELPLG(CD162) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SELPLG(CD162), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
应用 | FCM |
推荐剂量 | FCM: 1:25-1:100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PSGL-1/CD162 (rM PSGL-1/CD162; TMPY-03354; Q62170; Met1-Cys307). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin. |
偶联 | Unconjugated |
其他可选修饰符 | APC/FITC/PE |
可提供抗体种类 | 4 |
免疫原 | Recombinant Mouse PSGL-1/CD162 Protein (TMPY-03354) |
抗原种属 | Mouse |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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