Anti-Progesterone Receptor Antibody (8O274) is a Rabbit antibody targeting Progesterone Receptor. Anti-Progesterone Receptor Antibody (8O274) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

8O274

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Progesterone Receptor Monoclonal Antibody, Unconjugated (TMAB-11773) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. 25 ug total protein per lane of various lysates (see on figure) probed with Progesterone Receptor monoclonal antibody, unconjugated (TMAB-11773) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. The T-47D (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Progesterone Receptor antibody (TMAB-11773): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4. 4% Paraformaldehyde-fixed T-47D (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Progesterone Receptor) monoclonal Antibody, unconjugated (TMAB-11773) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM=1 μg/Test; ICC/IF=1:50-200; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

A synthesized peptide: human Progesterone receptor

Human

PGR

Progesterone receptor

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A is inactive in stimulating c-Src/MAPK signaling on hormone stimulation.