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Anti-Profilin-1 Antibody (1I976)
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产品编号 TMAC-03430
别名 Profilin-1, Profilin I, PFN1, Epididymis tissue protein Li 184a
Anti-Profilin-1 Antibody (1I976) 是一种 Rabbit 抗体，靶向 Profilin-1。Anti-Profilin-1 Antibody (1I976) 可用于 WB,ICC,IHC,FCM。
Anti-Profilin-1 Antibody (1I976)
Anti-Profilin-1 Antibody (1I976)
还可以产品编号 TMAC-03430 别名 Profilin-1, Profilin I, PFN1, Epididymis tissue protein Li 184a
Anti-Profilin-1 Antibody (1I976) 是一种 Rabbit 抗体，靶向 Profilin-1。Anti-Profilin-1 Antibody (1I976) 可用于 WB,ICC,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-Profilin-1 Antibody (1I976) is a Rabbit antibody targeting Profilin-1. Anti-Profilin-1 Antibody (1I976) can be used in WB,ICC,IHC,FCM. |
| 别名 | Profilin-1, Profilin I, PFN1, Epididymis tissue protein Li 184a |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin. 5. ICC staining Profilin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Profilin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Profilin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with Profilin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Profilins regulate Actin polymerization by binding to and sequestering the Actin monomer. Profilins act as a nucleotide exchange factor that charges Actin with ATP after binding the Actin monomer through a 1:1 stoichiometric relationship. Human Profilin-1 and Profilin-2 are encoded by two separate genes mapping to chromosomes 17p13.2 and 3q25.1, respectively. Both Profilin-1 and Profilin-2 are abundantly expressed in kidney. Profilin-1 is highly expressed in lung, liver, placenta and kidney while Profilin-2 is highly expressed in brain and skeletal muscle. In axonal and dendritic processes of mouse brain, Profilins co-localize with dynamin I and synapsin. Profilin may play a role in mediating cell adhesion. The overexpression of Profilin in endothelial cells results in increased adhesion to Fibronectin. In food allergy, plant Profilin is considered a pan-allergen. Case studies indicate individuals with allergies to various foods including celery, carrots, zucchini and peanuts are actually sensitive to the Profilin proteins in these foods. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 15 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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