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Anti-PRNP/Prion Antibody (2B223)
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产品编号 TMAC-03420
别名 PrPc, PrP33-35C, PrP27-30, PrP, PRIP, prion protein, p27-30, KURU, GSS, CJD, CD230, ASCR, AltPrP
Anti-PRNP/Prion Antibody (2B223) 是一种 Rabbit 抗体，靶向 PRNP/Prion。Anti-PRNP/Prion Antibody (2B223) 可用于 WB,ICC/IF,IHC,FCM。
Anti-PRNP/Prion Antibody (2B223)
Anti-PRNP/Prion Antibody (2B223)
还可以产品编号 TMAC-03420 别名 PrPc, PrP33-35C, PrP27-30, PrP, PRIP, prion protein, p27-30, KURU, GSS, CJD, CD230, ASCR, AltPrP
Anti-PRNP/Prion Antibody (2B223) 是一种 Rabbit 抗体，靶向 PRNP/Prion。Anti-PRNP/Prion Antibody (2B223) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PRNP/Prion Antibody (2B223) is a Rabbit antibody targeting PRNP/Prion. Anti-PRNP/Prion Antibody (2B223) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | PrPc, PrP33-35C, PrP27-30, PrP, PRIP, prion protein, p27-30, KURU, GSS, CJD, CD230, ASCR, AltPrP |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PrP on different lysates using anti-PrP antibody at 1/1,000 dilution. Positive control: Lane 1: Rat brain, Lane 2: Mouse brain. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PrP antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PrP antibody. Counter stained with hematoxylin. 4. ICC staining PrP in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining PrP in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining PrP in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of SH-SY-5Y cells with PrP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Prion diseases, or transmissible spongiform encephalopathies (TSEs), are manifested as genetic, infectious or sporadic, lethal neurodegenerative disorders involving alterations of the prion protein (PrP). Characteristic of prion diseases, cellular PrP (PrPc) is converted to the disease form, PrPSc, through alterations in the protein folding conformations. PrPc is constitutively expressed in normal adult brain and is sensitive to proteinase K digestion, while the altered PrPSc conformation is resistant to proteases, resulting in a distinct molecular mass after PK treatment. Consistent with the transient infection process of prion diseases, incubation of PrPc with PrPSc both in vitro and in vivo produces PrPc that is resistant to protease degradation. Infectious PrPSc is found at high levels in the brains of animals affected by TSEs, including scrapie in sheep, BSE in cattle and Cruetzfeldt-Jakob disease in humans. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 28 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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