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Anti-PRKN Antibody (1X34)
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产品编号 TMAC-03069
别名 PRKN, Parkinson juvenile disease protein 2 (Parkinson disease protein 2), Parkin RBR E3 ubiquitin-protein ligase, Parkin, PARK2, E3 ubiquitin-protein ligase parkin
Anti-PRKN Antibody (1X34) 是一种 Rabbit 抗体，靶向 PRKN。Anti-PRKN Antibody (1X34) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-PRKN Antibody (1X34)
Anti-PRKN Antibody (1X34)
还可以产品编号 TMAC-03069 别名 PRKN, Parkinson juvenile disease protein 2 (Parkinson disease protein 2), Parkin RBR E3 ubiquitin-protein ligase, Parkin, PARK2, E3 ubiquitin-protein ligase parkin
Anti-PRKN Antibody (1X34) 是一种 Rabbit 抗体，靶向 PRKN。Anti-PRKN Antibody (1X34) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PRKN Antibody (1X34) is a Rabbit antibody targeting PRKN. Anti-PRKN Antibody (1X34) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | PRKN, Parkinson juvenile disease protein 2 (Parkinson disease protein 2), Parkin RBR E3 ubiquitin-protein ligase, Parkin, PARK2, E3 ubiquitin-protein ligase parkin |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Parkin antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Parkin antibody. Counter stained with hematoxylin. 3. ICC staining Parkin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Parkin in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Parkin in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of SH-SY-5Y cells with Parkin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. 7. Western blot analysis of Parkin expression in Jurkat cell lysate. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200; IP: 1:50; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Parkin is a zinc-finger protein that is related to ubiquitin at the amino terminus. The wild type Parkin gene, which maps to human chromosome 6q25.2-27, encodes a 465 amino acid full-length protein that is expressed as multiple isoforms. Mutations in the Parkin gene are responsible for autosomal recessive juvenile Parkinson's disease and commonly involve deletions of exons 3-5. In humans, Parkin is expressed in a subset of cells of the basal ganglia, midbrain, cerebellum and cerebral cortex, and is subject to alternative splicing in different tissues. Parkin expression is also high in the brainstem of mice, with the majority of immunopositive cells being neurons. The Parkin gene has been identified in a diverse group of organisms including mammals, birds, frog and fruit flies, suggesting that analogous functional roles of the Parkin protein may have been highly conserved during the course of evolution. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 52 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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