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Anti-PRDM5 Antibody (2C105) 是一种 Mouse 抗体,靶向 PRDM5。Anti-PRDM5 Antibody (2C105) 可用于 WB,ICC,FCM。
Anti-PRDM5 Antibody (2C105) 是一种 Mouse 抗体,靶向 PRDM5。Anti-PRDM5 Antibody (2C105) 可用于 WB,ICC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,990 | 5日内发货 | |
100 μL | ¥ 2,995 | 5日内发货 |
产品描述 | Anti-PRDM5 Antibody (2C105) is a Mouse antibody targeting PRDM5. Anti-PRDM5 Antibody (2C105) can be used in WB,ICC,FCM. |
别名 | PRDM5_HUMAN, PRDM5 protein, PRDM 5, PR domain-containing protein 5, PR domain zinc finger protein 5, PR domain containing protein 5, PR domain containing 5, PFM2, PFM 2, BCS2 |
交叉反应 | Human,Mouse |
验证活性 | 1. Western blot analysis of PRDM5 on human PRDM5 recombinant protein using anti-PRDM5 antibody at 1/1,000 dilution. 2. Western blot analysis of PRDM5 on HEK293 (1) and PRDM5-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM5 antibody at 1/1,000 dilution. 3. ICC staining PRDM5 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of Hela cells with PRDM5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
应用 | |
推荐剂量 | WB: 1:1000-2000; ICC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | A cDNA of PRDM5 was isolated based upon its homology to the PR domain of PRDM2. The gene encodes an open reading frame of 630 amino acids and contains a PR domain in the NH-terminal region followed by 16 zinc finger motifs. Through radiation hybrid analysis, PRDM5 was mapped to human chromosome 4q27, a region thought to contain tumor suppressor genes for ovarian, breast, lung, liver, colon, and other cancers. The gene has a CpG island promoter and is silenced in human breast, ovarian, and liver cancers. Upon infection of tumor cells, a recombinant adenovirus expressing PRDM5 causes G2/M arrest and apoptosis, suggesting that inhibition of PRDM5 may be involved in carcinogenesis. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 73 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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