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Anti-PPM1A Antibody (9X611)
还可以产品编号 TMAY-02428
别名 protein phosphatase, Mg2+/Mn2+ dependent, 1A, PP2C-α, PP2Cα, PP2C-ALPHA, PP2Calpha, PP2CA
Anti-PPM1A Antibody (9X611) 是一种 Mouse 抗体，靶向 PPM1A。Anti-PPM1A Antibody (9X611) 可用于 FCM,ICC/IF。
Anti-PPM1A Antibody (9X611)
Anti-PPM1A Antibody (9X611)
还可以产品编号 TMAY-02428 别名 protein phosphatase, Mg2+/Mn2+ dependent, 1A, PP2C-α, PP2Cα, PP2C-ALPHA, PP2Calpha, PP2CA
Anti-PPM1A Antibody (9X611) 是一种 Mouse 抗体，靶向 PPM1A。Anti-PPM1A Antibody (9X611) 可用于 FCM,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,689 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-PPM1A Antibody (9X611) is a Mouse antibody targeting PPM1A. Anti-PPM1A Antibody (9X611) can be used in FCM,ICC/IF. |
| 别名 | protein phosphatase, Mg2+/Mn2+ dependent, 1A, PP2C-α, PP2Cα, PP2C-ALPHA, PP2Calpha, PP2CA |
| Ig Type | Monoclonal Mouse IgG2a |
| 克隆号 | 9X611 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human PPM1A in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human PPM1A monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human PPM1A expression on Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PPM1A, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PPM1A / PP2C-alpha (rh PPM1A / PP2C-alpha; TMPY-02567; P35813-1; Met 1-Trp 382). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | FITC/PE |
| 可提供抗体种类 | 3 |
| 免疫原 | Recombinant Human PPM1A / PP2C-alpha protein (TMPY-02567) |
| 抗原种属 | Human |
| 研究领域 | Phosphatases and Regulators |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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