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Anti-POU2F3 Polyclonal Antibody
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产品编号 TMAB-01561
Anti-POU2F3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 POU2F3。Anti-POU2F3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-POU2F3 Polyclonal Antibody
Anti-POU2F3 Polyclonal Antibody
还可以产品编号 TMAB-01561
Anti-POU2F3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 POU2F3。Anti-POU2F3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-POU2F3 Polyclonal Antibody is a Rabbit antibody targeting POU2F3. Anti-POU2F3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Mouse,Rat (predicted:Human,Dog,Cow,Rabbit,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (POU2F3) Polyclonal Antibody, Unconjugated (TMAB-01561) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Liver (Mouse) Lysate at 40 μg Primary: Anti-POU2F3 (TMAB-01561) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 48 kDa 3. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-POU2F3 (TMAB-01561) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 48 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011] |
| 免疫原 | KLH conjugated synthetic peptide: human POU2F3 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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