Anti-PODXL Polyclonal Antibody is a Rabbit antibody targeting PODXL. Anti-PODXL Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PCX Polyclonal Antibody, Unconjugated (TMAB-01558) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Kidney (Mouse) lysate at 30 μg;
Primary: Anti-PODXL (TMAB-01558) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (bse-0295G-HRP) at 1: 5000 dilution;
ECL excitated the fluorescence;
Predicted band size: 59 kDa
Observed band size:59 kDa
3. Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-PODXL antibody ,used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (TMAB-01558, 0.2 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of TMAB-01558 at 1/200 dilution for 30 min on ice.
4. Sample:
Liver (Mouse) Lysate at 40 μg
Primary: Anti-PODXL (TMAB-01558) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human PODXL

Human

Tumor biomarkers,Endothelial Cell Markers,Endothelium,Focal Adhesions,Surface Molecules