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Anti-Podocin Polyclonal Antibody

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Anti-Podocin Polyclonal Antibody
产品编号 TMAB-01556

Anti-Podocin Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Podocin。Anti-Podocin Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-Podocin Polyclonal Antibody

Anti-Podocin Polyclonal Antibody

Rating icon 还可以
Anti-Podocin Polyclonal Antibody
产品编号 TMAB-01556

Anti-Podocin Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Podocin。Anti-Podocin Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍

生物活性
产品描述
Anti-Podocin Polyclonal Antibody is a Rabbit antibody targeting Podocin. Anti-Podocin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. 25 μg total protein per Lane of various lysates probed with NPHS2 polyclonal antibody, unconjugated (TMAB-01556) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Polyclonal Antibody, Unconjugated (TMAB-01556) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Polyclonal Antibody, Unconjugated (TMAB-01556) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Peripheral membrane protein.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Podocin is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. It is a hairpin like integral membrane protein with intracellular N and C termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
抗原信息
免疫原
KLH conjugated synthetic peptide: human NPHS2/Podocin
抗原种属
Human
基因ID
Uniprot ID
研究领域
Other Antibodies,Transmembrane,Other Cell Types
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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