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Anti-PLCγ1 Antibody (8G514)
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产品编号 TMAC-03370
别名 PLCy1, PLC-II, PLCgamma1, PLC148, PLC1, PLC γ1, PLC γ 1, PLC y1, PLC y 1, PLC gamma1, PLC gamma 1, NCKAP3, IDAA
Anti-PLCγ1 Antibody (8G514) 是一种 Mouse 抗体，靶向 PLCγ1。Anti-PLCγ1 Antibody (8G514) 可用于 WB,ICC,IHC,FCM。
Anti-PLCγ1 Antibody (8G514)
Anti-PLCγ1 Antibody (8G514)
还可以产品编号 TMAC-03370 别名 PLCy1, PLC-II, PLCgamma1, PLC148, PLC1, PLC γ1, PLC γ 1, PLC y1, PLC y 1, PLC gamma1, PLC gamma 1, NCKAP3, IDAA
Anti-PLCγ1 Antibody (8G514) 是一种 Mouse 抗体，靶向 PLCγ1。Anti-PLCγ1 Antibody (8G514) 可用于 WB,ICC,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,990 | 5日内发货 | |
| 100 μL | ¥ 2,985 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PLCγ1 Antibody (8G514) is a Mouse antibody targeting PLCγ1. Anti-PLCγ1 Antibody (8G514) can be used in WB,ICC,IHC,FCM. |
| 别名 | PLCy1, PLC-II, PLCgamma1, PLC148, PLC1, PLC γ1, PLC γ 1, PLC y1, PLC y 1, PLC gamma1, PLC gamma 1, NCKAP3, IDAA |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PLCG1 on human PLCG1 recombinant protein using anti-PLCG1 antibody at 1/1,000 dilution. 2. Western blot analysis of PLCG1 on HEK293 (1) and PLCG1-hIgGFc transfected HEK293 (2) cell lysate using anti-PLCG1 antibody at 1/1,000 dilution. 3. Western blot analysis of PLCG1 on different cell lysate using anti-PLCG1 antibody at 1/1,000 dilution. Positive control: Line1: Hela, Line2: A431, Line3: C6, Line4: NIH/3T3, Line5: COS7, Line6: HCT116. 4. Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin. 6. ICC staining PLCG1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Jurkat cells with PLCG1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:100-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Phospholipase C-gamma 1 (PLC g1) is an isozyme of the phosphoinositide-specific PLC family, which occupies a central role in hormonal signal transduction pathways and is a substrate for the epidermal growth factor receptor tyrosine kinase. Following activation of TrkB, PLC g1 is phosphorylated on Tyrosine 783, Tyrosine 771 and Tyrosine 1253. Tyrosine 783 lies just downstream of the kinase domain in a relatively short sequence motif characteristic of the Trk family of protein-tyrosine kinase receptors. The sequence around Tyrosine 783 fits a consensus sequence for binding PLC g1. PLC g1 also forms a complex with TrkB consistent with the possibility that one of the TrkB autophosphorylation sites provides a binding site for the PLC g1 SH2 domains, as is the case for other receptor protein-tyrosine kinases. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 148 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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