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Anti-PKC beta Antibody (5T436)
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产品编号 TMAC-03314
别名 Protein kinase C beta type, Protein kinase C beta, PRKCB2, PRKCB II, Prkcb, PKCβ, PKCbeta, PKC-B, PKCB, PKC β, PKC Beta, PKC B, KPCB_HUMAN
Anti-PKC beta Antibody (5T436) 是一种 Rabbit 抗体，靶向 PKC beta。Anti-PKC beta Antibody (5T436) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-PKC beta Antibody (5T436)
Anti-PKC beta Antibody (5T436)
还可以产品编号 TMAC-03314 别名 Protein kinase C beta type, Protein kinase C beta, PRKCB2, PRKCB II, Prkcb, PKCβ, PKCbeta, PKC-B, PKCB, PKC β, PKC Beta, PKC B, KPCB_HUMAN
Anti-PKC beta Antibody (5T436) 是一种 Rabbit 抗体，靶向 PKC beta。Anti-PKC beta Antibody (5T436) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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偶联与修饰
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| 产品描述 | Anti-PKC beta Antibody (5T436) is a Rabbit antibody targeting PKC beta. Anti-PKC beta Antibody (5T436) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | Protein kinase C beta type, Protein kinase C beta, PRKCB2, PRKCB II, Prkcb, PKCβ, PKCbeta, PKC-B, PKCB, PKC β, PKC Beta, PKC B, KPCB_HUMAN |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PKC beta 2 on Raji cell lysates using anti-PKC beta 2 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin. 5. ICC staining PKC beta 2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with PKC beta 2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional PKC isoforms (α, βI, βII and γ) and novel PKC isoforms. Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ?? are independent of Ca2+. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 77 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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