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Anti-PKA 2 beta Antibody (8T490)
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产品编号 TMAC-03306
别名 WUGSC:H RG363E19.2, RIIbeta, RII(beta), RII beta, RATDNA, Protein kinase cAMP dependent regulatory type II beta, PRKAR2B, PRKAR2, PRKAR 2, Pkarb2, PKA2β, PKA2beta, PKA2b, PKA 2 β, PKA 2 b, MGC116401, KAP3_HUMAN, H RG363E19.2, cAMP-dependent protein kinase type II-beta regulatory subunit, cAMP dependent protein kinase type II beta regulatory subunit, cAMP dependent protein kinase type II beta regulatory chain, AW061005, AI451071
Anti-PKA 2 beta Antibody (8T490) 是一种 Rabbit 抗体，靶向 PKA 2 beta。Anti-PKA 2 beta Antibody (8T490) 可用于 WB,IP,ICC/IF,IHC,FCM。
Anti-PKA 2 beta Antibody (8T490)
Anti-PKA 2 beta Antibody (8T490)
还可以产品编号 TMAC-03306 别名 WUGSC:H RG363E19.2, RIIbeta, RII(beta), RII beta, RATDNA, Protein kinase cAMP dependent regulatory type II beta, PRKAR2B, PRKAR2, PRKAR 2, Pkarb2, PKA2β, PKA2beta, PKA2b, PKA 2 β, PKA 2 b, MGC116401, KAP3_HUMAN, H RG363E19.2, cAMP-dependent protein kinase type II-beta regulatory subunit, cAMP dependent protein kinase type II beta regulatory subunit, cAMP dependent protein kinase type II beta regulatory chain, AW061005, AI451071
Anti-PKA 2 beta Antibody (8T490) 是一种 Rabbit 抗体，靶向 PKA 2 beta。Anti-PKA 2 beta Antibody (8T490) 可用于 WB,IP,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PKA 2 beta Antibody (8T490) is a Rabbit antibody targeting PKA 2 beta. Anti-PKA 2 beta Antibody (8T490) can be used in WB,IP,ICC/IF,IHC,FCM. |
| 别名 | WUGSC:H RG363E19.2, RIIbeta, RII(beta), RII beta, RATDNA, Protein kinase cAMP dependent regulatory type II beta, PRKAR2B, PRKAR2, PRKAR 2, Pkarb2, PKA2β, PKA2beta, PKA2b, PKA 2 β, PKA 2 b, MGC116401, KAP3_HUMAN, H RG363E19.2, cAMP-dependent protein kinase type II-beta regulatory subunit, cAMP dependent protein kinase type II beta regulatory subunit, cAMP dependent protein kinase type II beta regulatory chain, AW061005, AI451071 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PKA 2 beta (regulatory subunit) antibody. Counter stained with hematoxylin. 2. ICC staining PKA 2 beta (regulatory subunit) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining PKA 2 beta (regulatory subunit) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining PKA 2 beta (regulatory subunit) in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of N2A cells with PKA 2 beta (regulatory subunit) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The second messenger cyclic AMP mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. One of several regulatory subunits, p-PKA IIβ reg (cAMP-dependent protein kinase type II-beta regulatory subunit), also known as PRKAR2B, is a 418 amino acid protein that is phosphorylated by the activated catalytic chain. p-PKA IIβ reg knockout mice exhibit diminished white adipose tissue and were protected against diet-induced obesity and fatty livers,as well as markedly reduced leptin mRNA. Also playing a role in the immune response, p-PKA IIβ reg suppresses CREB transcriptional activity and down-regulates IL-2 production in T-lymphocytes. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 46 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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