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顶部生物活性储存和溶解信息计算器

Anti-PIST Antibody (5T628)

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Anti-PIST Antibody (5T628)
产品编号 TMAC-03304
别名 PIST, GOPC1, golgi-associated PDZ and coiled-coil motif containing, FIG, dJ94G16.2, CAL

Anti-PIST Antibody (5T628) 是一种 Rabbit 抗体，靶向 PIST。Anti-PIST Antibody (5T628) 可用于 WB,ICC,IF,IP,IHC,FCM。

Anti-PIST Antibody (5T628)

Anti-PIST Antibody (5T628)

Rating icon 还可以
Anti-PIST Antibody (5T628)
产品编号 TMAC-03304 别名 PIST, GOPC1, golgi-associated PDZ and coiled-coil motif containing, FIG, dJ94G16.2, CAL

Anti-PIST Antibody (5T628) 是一种 Rabbit 抗体，靶向 PIST。Anti-PIST Antibody (5T628) 可用于 WB,ICC,IF,IP,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-PIST Antibody (5T628) is a Rabbit antibody targeting PIST. Anti-PIST Antibody (5T628) can be used in WB,ICC,IF,IP,IHC,FCM.
别名PIST, GOPC1, golgi-associated PDZ and coiled-coil motif containing, FIG, dJ94G16.2, CAL
Ig Type
IgG
交叉反应
Human,Mouse
验证活性
1. Western blot analysis of PIST on SiHa cell using anti-PIST antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PIST antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PIST antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PIST antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PIST antibody. Counter stained with hematoxylin.
6. ICC staining PIST in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining PIST in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining PIST in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
应用
WB
ICC
IF
IP
IHC
FCM
推荐剂量
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景PIST (PDZ protein interacting specifically with TC10), also known as GOPC (golgi associated PDZ and coiled-coil motif containing), CAL or FIG, is a 462 amino acid protein that localizes to the cytoplasm, as well as to the membrane of the golgi apparatus and to the cell junction. Expressed ubiquitously and containing one PDZ (DHR) domain, PIST functions as a homooligomer that interacts with a variety of proteins and plays a role in intracellular protein trafficking and degradation. Additionally, PIST is thought to regulate ionic currents via membrane channel modification and may also play a role in autophagy. Chromosomal aberrations in the gene encoding PIST are found in glioblastoma multiform (GBM), a common and aggressive form of brain tumor, suggesting a role for mutated PIST in carcinogenesis. Three isoforms of PIST exist due to alternative splicing events.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: human PIST aa 1-100
抗原种属
human
Uniprot ID
Q9HD26
化学信息
分子量Theoretical: 51 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

GOPC-1GOPC 1

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