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Anti-PHPT1 Antibody (2C534)
还可以产品编号 TMAY-02359
Anti-PHPT1 Antibody (2C534) 是一种 Rabbit 抗体，靶向 PHPT1。Anti-PHPT1 Antibody (2C534) 可用于 IHC-P,FCM,ICC/IF。
Anti-PHPT1 Antibody (2C534)
Anti-PHPT1 Antibody (2C534)
还可以产品编号 TMAY-02359
Anti-PHPT1 Antibody (2C534) 是一种 Rabbit 抗体，靶向 PHPT1。Anti-PHPT1 Antibody (2C534) 可用于 IHC-P,FCM,ICC/IF。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-PHPT1 Antibody (2C534) is a Rabbit antibody targeting PHPT1. Anti-PHPT1 Antibody (2C534) can be used in IHC-P,FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 2C534 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunochemical staining of human PHPT1 in human skeletal muscle with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). 2. Flow cytometric analysis of Human PHPT1 expression on THP1 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PHPT1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 3. Immunofluorescence staining of Human PHPT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PHPT1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. |
| 应用 | IHC-P,FCM,ICC/IF |
| 推荐剂量 | IHC-P: 1:2500-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human PHPT1 (rh PHPT1; TMPY-02002; Q9NRX4-1; Ala2-Tyr125). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | PHPT1, also known as 14 kDa phosphohistidine phosphatase, phosphohistidine phosphatase 1, protein janus-A homolog, PHP14, is a cytoplasm protein which belongs to the janus family. PHPT1 / PHP14 is expressed abundantly in heart and skeletal muscle. Phosphatases are a diverse group of enzymes that regulate numerous cellular processes. Much of what is known relates to the tyrosine, threonine, and serine phosphatases, whereas the histidine phosphatases have not been studied as much. Protein histidine phosphorylation exists widely in vertebrates, and it plays important roles in signal transduction and other cellular functions. Protein histidine phosphorylation accounts for about 6% of the total protein phosphorylation in eukaryotic cells. The knowledge about eukaryotic PHPT (protein histidine phosphatase) is still very limited. To date, only one vertebrate PHPT has been discovered, and two crystal structures of human PHPT1 have been solved. PHPT1 / PHP14 can dephosphorylate a variety of proteins (e.g. ATP-citrate lyase and the beta-subunit of G proteins). A putative active site has been identified by its electrostatic character, ion binding, and conserved protein residues. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human PHPT1 protein (TMPY-02002) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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