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Anti-Phosphotyrosine Antibody (1A392) 是一种 Mouse 抗体,靶向 Phosphotyrosine。Anti-Phosphotyrosine Antibody (1A392) 可用于 IHC-P,IHC-Fr,IF。
Anti-Phosphotyrosine Antibody (1A392) 是一种 Mouse 抗体,靶向 Phosphotyrosine。Anti-Phosphotyrosine Antibody (1A392) 可用于 IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
| 产品描述 | Anti-Phosphotyrosine Antibody (1A392) is a Mouse antibody targeting Phosphotyrosine. Anti-Phosphotyrosine Antibody (1A392) can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 1A392 |
| 反应种属 | Species independent |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated (TMAB-11407) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated (TMAB-11407) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated (TMAB-11407) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated (TMAB-11407) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated (TMAB-11407) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
| 应用 | IHC-PIHC-FrIF |
| 推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function. |
| 免疫原 | KLH conjugated Phosphotyrosine |
| 基因ID |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |