Anti-Phospho-YAP1 (Tyr407) Polyclonal Antibody is a Rabbit antibody targeting Phospho-YAP1 (Tyr407). Anti-Phospho-YAP1 (Tyr407) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YAP1 (Tyr407)) Polyclonal Antibody, Unconjugated (TMAB-01532) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat breast tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YAP1 (Tyr407)) Polyclonal Antibody, Unconjugated (TMAB-01532) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-YAP1 (Tyr407)) Polyclonal Antibody, Unconjugated (TMAB-01532) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-YAP1 (Tyr407)) Polyclonal Antibody, Unconjugated (TMAB-01532) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-YAP1 (Tyr407)) Polyclonal Antibody, Unconjugated (TMAB-01532) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 μg
Primary: Anti-Phospho-YAP1 (Tyr407) (TMAB-01532) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65-70 kDa
Observed band size: 70 kDa
7. Sample:
Hela (Human) Cell Lysate at 30 μg
Hela-Uv (Human) Cell Lysate at 30 μg
293t (Human) Cell Lysate at 30 μg
293T-Uv (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-YAP1 (Tyr407) (TMAB-01532) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65-70 kDa
Observed band size: 70 kDa
8. Sample:
Lung (Mouse) Lysate at 40 μg
Lung (Rat) Lysate at 40 μg
Primary: Anti-Phospho-YAP1 (Tyr407) (TMAB-01532) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65-70 kDa
Observed band size: 70 kDa
9. Paraformaldehyde-fixed, paraffin embedded (human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-YAP1 (Tyr407)) Polyclonal Antibody, Unconjugated (TMAB-01532) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-YAP1 (Tyr407)) Polyclonal Antibody, Unconjugated (TMAB-01532) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human YAP around the phosphorylation site of Tyr407

Human

Signal transduction,Proto-oncogenes,Transcription Factors,Cytoplasmic,Src Family