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Anti-Phospho-STAT3 (Ser727) Antibody (9W152)

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Anti-Phospho-STAT3 (Ser727) Antibody (9W152)
产品编号 TMAC-03275
别名 STAT3 (p-Ser727), STAT3 (p-S727), p-STAT3 (Ser727), p-STAT3 (S727)

Anti-Phospho-STAT3 (S727) Antibody (9W152) 是一种 Rabbit 抗体,靶向 Phospho-STAT3 (S727)。Anti-Phospho-STAT3 (S727) Antibody (9W152) 可用于 WB,IHC,ICC/IF,IP。

Anti-Phospho-STAT3 (Ser727) Antibody (9W152)

Anti-Phospho-STAT3 (Ser727) Antibody (9W152)

Rating icon 还可以
Anti-Phospho-STAT3 (Ser727) Antibody (9W152)
产品编号 TMAC-03275 别名 STAT3 (p-Ser727), STAT3 (p-S727), p-STAT3 (Ser727), p-STAT3 (S727)

Anti-Phospho-STAT3 (S727) Antibody (9W152) 是一种 Rabbit 抗体,靶向 Phospho-STAT3 (S727)。Anti-Phospho-STAT3 (S727) Antibody (9W152) 可用于 WB,IHC,ICC/IF,IP。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-STAT3 (Ser727) Antibody (9W152) 是一种 Rabbit 抗体,靶向 Phospho-STAT3 (Ser727)。Anti-Phospho-STAT3 (Ser727) Antibody (9W152) 可用于 WB,IHC,ICC/IF,IP。
别名STAT3 (p-Ser727), STAT3 (p-S727), p-STAT3 (Ser727), p-STAT3 (S727)
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Phospho-STAT3 (S727) on different lysates using anti-Phospho-STAT3 (S727) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: NIH/3T3.
2. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Phospho-STAT3 (S727) antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-STAT3 (S727) antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3 (S727) antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Phospho-STAT3 (S727) antibody. Counter stained with hematoxylin.
6. ICC staining Phospho-STAT3 (S727) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Phospho-STAT3 (S727) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining Phospho-STAT3 (S727) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
A synthesized phosphopeptide: human STAT3 around the phosphorylation site of Ser727
抗原种属
human
Uniprot ID
化学信息
分子量Theoretical: 88 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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