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顶部生物活性储存和溶解信息计算器

Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody

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Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody
产品编号 TMAB-01497
别名 Smad2/3 (p-Thr8), Smad2/3 (p-T8), SMAD family member 3, SMAD family member 2, SMAD 3, SMAD 2, SMA and MAD related protein 3, Sma and Mad related protein 2, p-Smad2/3 (Thr8), p-Smad2/3 (T8), Mothers against DPP homolog 3, Mothers against DPP homolog 2, MADR2, MADH3, MADH2, Mad related protein 2, hSMAD3, hSMAD2, hMAD 3, hMAD 2

Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-Smad2/3 (Thr8)。Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody 可用于 WB,FCM。

Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody

Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody

Rating icon 还可以
Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody
产品编号 TMAB-01497 别名 Smad2/3 (p-Thr8), Smad2/3 (p-T8), SMAD family member 3, SMAD family member 2, SMAD 3, SMAD 2, SMA and MAD related protein 3, Sma and Mad related protein 2, p-Smad2/3 (Thr8), p-Smad2/3 (T8), Mothers against DPP homolog 3, Mothers against DPP homolog 2, MADR2, MADH3, MADH2, Mad related protein 2, hSMAD3, hSMAD2, hMAD 3, hMAD 2

Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-Smad2/3 (Thr8)。Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody 可用于 WB,FCM。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Smad2/3 (Thr8). Anti-Phospho-Smad2/3 (Thr8) Polyclonal Antibody can be used in WB,FCM.
别名Smad2/3 (p-Thr8), Smad2/3 (p-T8), SMAD family member 3, SMAD family member 2, SMAD 3, SMAD 2, SMA and MAD related protein 3, Sma and Mad related protein 2, p-Smad2/3 (Thr8), p-Smad2/3 (T8), Mothers against DPP homolog 3, Mothers against DPP homolog 2, MADR2, MADH3, MADH2, Mad related protein 2, hSMAD3, hSMAD2, hMAD 3, hMAD 2
Ig Type
IgG
交叉反应
Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse)
验证活性
1. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-phospho-Smad2/Smad3 (Thr8) antibody (TMAB-01497)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. blank: A549 cells (blue line)
isotype control: rabbit IgG (orange line)
second antibody: goat anti-rabbit IgG (white blue line)
primary antibody: rabbit Anti-phospho-Smad2/Smad3 (Thr8) (green line); contration: 3 μg/10^6 cells
3. Sample:
HUVEC cell (human) Lysate at 30 μg
Primary: Anti-p-Smad2/Smad3 (Thr8) (TMAB-01497) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
4. Sample:
cerebrum (mouse) Lysate at 40 μg
RSC96 cell (rat) Lysate at 30 μg
hepG2 cell (human) Lysate at 30 μg
Hcclm3 cell (human) Lysate at 30 μg
Primary: Anti-p-Smad2/Smad3 (Thr8) (TMAB-01497) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 48,52 kDa
5. Sample:
U251 cell (human) Lysate at 30 μg
Primary: Anti-p-Smad2/Smad3 (Thr8) (TMAB-01497) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
应用
WB
FCM
推荐剂量
WB: 1:500-2000; FCM: 1μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
抗原信息
免疫原
KLH conjugated synthesised phosphopeptide: human Smad2/Smad3 around the phosphorylation site of Thr8
抗原种属
Human
基因ID
4087
Uniprot ID
Q15796
研究领域
TGF,SMADs,SMADs,Cytoplasmic
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

  • 稀释 计算器

关键词

Smad2/3 (pThr8)Smad2/3 (pT8)Smad2/3 (phospho-Thr8)Smad2/3 (phospho-T8)pSmad2/3 (Thr8)pSmad2/3 (T8)

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