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Anti-Phospho-POLR2A (Ser5) Antibody (4B857)
还可以产品编号 TMAH-00942
别名 RPOL2, RPO2, RpIILS, RPBh1, RPB1, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, Polymerase (RNA) II (DNA directed) polypeptide A 220kDa, POLRA, Polr2a, POLR2, Phospho-POLR2A (S5), hsRPB1, hRPB220, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit, DNA directed RNA polymerase II A
Anti-Phospho-POLR2A (Ser5) Antibody (4B857) 是一种抗体，靶向 Phospho-POLR2A (Ser5)。Anti-Phospho-POLR2A (Ser5) Antibody (4B857) 可用于 ELISA, WB, IHC, IF。
Anti-Phospho-POLR2A (Ser5) Antibody (4B857)
Anti-Phospho-POLR2A (Ser5) Antibody (4B857)
还可以产品编号 TMAH-00942 别名 RPOL2, RPO2, RpIILS, RPBh1, RPB1, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, Polymerase (RNA) II (DNA directed) polypeptide A 220kDa, POLRA, Polr2a, POLR2, Phospho-POLR2A (S5), hsRPB1, hRPB220, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit, DNA directed RNA polymerase II A
Anti-Phospho-POLR2A (Ser5) Antibody (4B857) 是一种抗体，靶向 Phospho-POLR2A (Ser5)。Anti-Phospho-POLR2A (Ser5) Antibody (4B857) 可用于 ELISA, WB, IHC, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-POLR2A (Ser5) Antibody (4B857) is an antibody targeting Phospho-POLR2A (Ser5). Anti-Phospho-POLR2A (Ser5) Antibody (4B857) can be used in ELISA, WB, IHC, IF. |
| 别名 | RPOL2, RPO2, RpIILS, RPBh1, RPB1, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, Polymerase (RNA) II (DNA directed) polypeptide A 220kDa, POLRA, Polr2a, POLR2, Phospho-POLR2A (S5), hsRPB1, hRPB220, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit, DNA directed RNA polymerase II A |
| Ig Type | Rabbit IgG |
| 克隆号 | 4B857 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysate -All lanes Phospho-POLR2A antibody at 0.75μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 270 KDa -Observed band size: 270 KDa 2. Immunocytochemistry analysis of TMAH-00942 diluted at 1:75 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. Immunofluorescence staining of Hela cells with TMAH-00942 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, WB, IHC, IF |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Cytoplasm. Chromosome. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines. Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD. Methylation and dimethylation have a repressive effect on target genes expression. (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Phospho-POLR2A (S5) |
| 抗原种属 | Human |
| 基因ID | 5430 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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