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Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2

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Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2
产品编号 TMAB-01477
别名 WRS, PRKR-like endoplasmic reticulum kinase, p-PERK (Thr980), p-PERK (T980), PERK (p-Thr980), PERK (p-T980), PEK, Pancreatic eIF2-alpha kinase, Pancreatic eIF2 alpha kinase, HsPEK, HRI, Heme regulated EIF2 alpha kinase, Eukaryotic translation initiation factor 2 alpha kinase 3, EIF2AK3, EC 2.7.11.1, E2AK3, DKFZp781H1925

Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 Phospho-PERK (Thr980)。Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF,FCM。

Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2

Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2

Rating icon 还可以
Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2
产品编号 TMAB-01477 别名 WRS, PRKR-like endoplasmic reticulum kinase, p-PERK (Thr980), p-PERK (T980), PERK (p-Thr980), PERK (p-T980), PEK, Pancreatic eIF2-alpha kinase, Pancreatic eIF2 alpha kinase, HsPEK, HRI, Heme regulated EIF2 alpha kinase, Eukaryotic translation initiation factor 2 alpha kinase 3, EIF2AK3, EC 2.7.11.1, E2AK3, DKFZp781H1925

Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 Phospho-PERK (Thr980)。Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF,FCM。

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50 μL
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100 μL
¥ 1,970
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¥ 2,790
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产品介绍

生物活性
产品描述
Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-PERK (Thr980). Anti-Phospho-PERK (Thr980) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.
别名WRS, PRKR-like endoplasmic reticulum kinase, p-PERK (Thr980), p-PERK (T980), PERK (p-Thr980), PERK (p-T980), PEK, Pancreatic eIF2-alpha kinase, Pancreatic eIF2 alpha kinase, HsPEK, HRI, Heme regulated EIF2 alpha kinase, Eukaryotic translation initiation factor 2 alpha kinase 3, EIF2AK3, EC 2.7.11.1, E2AK3, DKFZp781H1925
Ig Type
IgG
交叉反应
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERk (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01477) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01477) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01477) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Blank control: A431. Primary Antibody (green line): Rabbit Anti-PERk (Thr980) antibody (TMAB-01477)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Blank control (Black line): A431 (Black).
Primary Antibody (green line): Rabbit Anti-PERk (Thr980) antibody (TMAB-01477)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at-20°C. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
6. Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (TMAB-01477) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-Phospho-PERK (Thr980) antibody (TMAB-01477)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
8. Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human MCF-7-Tg (1 μM thapsigargin, 20 min) cell lysates
Lane 3: Human MCF-7-phosphatase cell lysates
Primary: Anti-Phospho-PERK (Thr980) (TMAB-01477) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 120 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Endoplasmic reticulum membrane; Single-pass type I membrane protein.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010].
抗原信息
免疫原
KLH conjugated synthesised phosphopeptide: mouse PERK around the phosphorylation site of Thr980
抗原种属
Mouse
基因ID
Uniprot ID
研究领域
Regulation,Eukaryotic Initiation factors (eIF's),Diabetes,Metabolic disorders,Obesity
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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