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Anti-Phospho-NFKBIA (Ser32) Antibody (5H611)
还可以产品编号 TMAH-00936
别名 Phospho-NFKBIA (S32), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) 是一种抗体，靶向 Phospho-NFKBIA (Ser32)。Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) 可用于 ELISA, WB, IF。
Anti-Phospho-NFKBIA (Ser32) Antibody (5H611)
Anti-Phospho-NFKBIA (Ser32) Antibody (5H611)
还可以产品编号 TMAH-00936 别名 Phospho-NFKBIA (S32), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) 是一种抗体，靶向 Phospho-NFKBIA (Ser32)。Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) 可用于 ELISA, WB, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) is an antibody targeting Phospho-NFKBIA (Ser32). Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) can be used in ELISA, WB, IF. |
| 别名 | Phospho-NFKBIA (S32), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α |
| Ig Type | Rabbit IgG |
| 克隆号 | 5H611 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in A549 whole cell lysate(treated with Calyculin A or not) -All lanes Phospho-NFKBIA antibody at 1.07μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 39 KDa -Observed band size: 39 KDa 2. Immunofluorescence staining of A549 cells with TMAH-00936 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, WB, IF |
| 推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Phospho-NFKBIA (S32) |
| 抗原种属 | Human |
| 基因ID | 4792 |
| Uniprot ID | |
| 研究领域 | Signal Transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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