Anti-Phospho-MST1 /2 (Thr183, 180) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MST1 /2 (Thr183, 180). Anti-Phospho-MST1 /2 (Thr183, 180) Polyclonal Antibody can be used in WB.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Cow,Horse,Rabbit)

1. Sample:
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-Mst1 (Thr183) + Mst2 (Thr180) (TMAB-01457) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/37/56 kDa
Observed band size: 37 kDa
2. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-Mst1 (Thr183) + Mst2 (Thr180) (TMAB-01457) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/37/56 kDa
Observed band size: 37 kDa
3. Sample:
293t (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-Mst1 (Thr183) + Mst2 (Thr180) (TMAB-01457) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/37/56 kDa
Observed band size: 37 kDa

WB: 1:500-2000

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Mst1 around the phosphorylation site of Thr183

Human

STK4

Serine/threonine-protein kinase 4

Apoptosis,Hypertrophy,Other Kinases

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.