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顶部生物活性储存和溶解信息计算器

Anti-Phospho-JNK1/2/MAPK10 (Thr183/183/221) Antibody (7N200)

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产品编号 TMAC-03232

别名 p-JNK1/2/MAPK10 (Thr183/183/221), p-JNK1/2/MAPK10 (T183/183/221), JNK1/2/MAPK10 (p-Thr183/183/221), JNK1/2/MAPK10 (p-T183/183/221)

Anti-Phospho-JNK1/2/MAPK10 (Thr183/183/221) Antibody (7N200) 是一种 Rabbit 抗体，靶向 Phospho-JNK1/2/MAPK10 (Thr183/183/221)。Anti-Phospho-JNK1/2/MAPK10 (Thr183/183/221) Antibody (7N200) 可用于 WB,ICC/IF,IHC,IP,FCM。

Anti-Phospho-JNK1/2/MAPK10 (Thr183/183/221) Antibody (7N200)

Anti-Phospho-JNK1/2/MAPK10 (Thr183/183/221) Antibody (7N200)

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产品编号 TMAC-03232 别名 p-JNK1/2/MAPK10 (Thr183/183/221), p-JNK1/2/MAPK10 (T183/183/221), JNK1/2/MAPK10 (p-Thr183/183/221), JNK1/2/MAPK10 (p-T183/183/221)

Anti-Phospho-JNK1/2/MAPK10 (Thr183/183/221) Antibody (7N200) 是一种 Rabbit 抗体，靶向 Phospho-JNK1/2/MAPK10 (Thr183/183/221)。Anti-Phospho-JNK1/2/MAPK10 (Thr183/183/221) Antibody (7N200) 可用于 WB,ICC/IF,IHC,IP,FCM。

规格价格库存数量
50 μL
¥ 1,485
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100 μL
¥ 2,495
5日内发货
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生物活性
产品描述
Anti-Phospho-JNK1/2/MAPK10 (Thr183/183/221) Antibody (7N200) is a Rabbit antibody targeting Phospho-JNK1/2/MAPK10 (Thr183/183/221). Anti-Phospho-JNK1/2/MAPK10 (Thr183/183/221) Antibody (7N200) can be used in FCM,ICC/IF,IHC,IP,WB.
别名p-JNK1/2/MAPK10 (Thr183/183/221), p-JNK1/2/MAPK10 (T183/183/221), JNK1/2/MAPK10 (p-Thr183/183/221), JNK1/2/MAPK10 (p-T183/183/221)
Ig Type
IgG
克隆号
7N200
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Phospho-JNK1/2/3 (T183+T183+T221) on different lysates using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 cell lysate, treated with Anisomycin, Lane 2: NIH/3T3 cell lysate, untreated.
2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.
5. ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Hela cells with Phospho-JNK1/2/3 (T183+T183+T221) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用FCMICC/IFIHCIPWB
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
A synthesized phosphopeptide: human JNK1/2/3 around the phosphorylation site of Thr183 + Thr183 + Thr221
抗原种属
human
化学信息
分子量Theoretical: 54/46 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

pJNK1/2/MAPK10 (Thr183/183/221)pJNK1/2/MAPK10 (T183/183/221)JNK1/2/MAPK10 (pThr183/183/221)JNK1/2/MAPK10 (pT183/183/221)JNK1/2/MAPK10 (phospho-Thr183/183/221)JNK1/2/MAPK10 (phospho-T183/183/221)

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