Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Tyr42). Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse (predicted:Pig,Cow,Rabbit,Sheep)

1. Blank control (blue line): Jurkat (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-phospho-IKB alpha (Tyr42) antibody (TMAB-01429), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test.
2. Blank control (blue line): Hela (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C).
Primary Antibody (green line): Rabbit Anti-phospho-IKB alpha (Tyr42) antibody (TMAB-01429), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
3. Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 μg
Lane 2: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-IKB alpha (Tyr42) (TMAB-01429) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 37 kDa
4. Sample:
Thymus (Mouse) Lysate at 40 μg
Primary: Anti-phospho-IKB alpha (Tyr42) (TMAB-01429) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
5. Sample:
Raji (Human) Lysate at 40 μg
Primary: Anti-phospho-IKB alpha (Tyr42) (TMAB-01429) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
6. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-IKB alpha (Tyr42)) Polyclonal Antibody, Unconjugated (TMAB-01429) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human IKB alpha around the phosphorylation site of Tyr42

Human

NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway