Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693) is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser32, 36). Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693) can be used in IF,IHC-Fr,IHC-P,WB.

IgG

7S693

Human,Mouse,Rat

1. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:2000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): HeLa, (+): HeLa+ Human α-TNF (20ng/ml, 30 min)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 36 kDa
Observed MW: 36 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:2000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): C6, (+): C6+ Calyculin A (100nM, 30 min)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 36 kDa
Observed MW: 36 kDa
3. Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50 U/ml) at 37°C for 1 hour
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Secondary: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-01425
4. Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline
phosphatase (50 U/ml) at 37°C for 1 hour
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Secondary: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-01425

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:50-100; IF: 1:1:50-100

Monoclonal

KLH conjugated synthetic peptide: human phospho-IKB Alpha (Ser32 / Ser36)

Human

NFKBIA

NF-kappa-B inhibitor alpha

NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.