Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693) is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser32, 36). Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

7S693

Human,Mouse,Rat

1. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:2000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): HeLa, (+): HeLa+ Human α-TNF (20ng/ml, 30 min)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 36 kDa
Observed MW: 36 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:2000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): C6, (+): C6+ Calyculin A (100nM, 30 min)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 36 kDa
Observed MW: 36 kDa
3. Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50 U/ml) at 37°C for 1 hour
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Secondary: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-01425
4. Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline
phosphatase (50 U/ml) at 37°C for 1 hour
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0
Primary dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Secondary: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-01425

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:50-100; IF: 1:1:50-100

Monoclonal

KLH conjugated synthetic peptide: human phospho-IKB Alpha (Ser32 / Ser36)

Human

NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway