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Anti-Phospho-HSF1 (S326) Antibody (5H836)
还可以产品编号 TMAH-00909
别名 Phospho-HSF1 (S326), HSTF1, heat shock transcription factor 1
Anti-Phospho-HSF1 (Ser362) Antibody (5H836) 是一种抗体，靶向 Phospho-HSF1 (Ser362)。Anti-Phospho-HSF1 (Ser362) Antibody (5H836) 可用于 ELISA, WB, ICC。
Anti-Phospho-HSF1 (S326) Antibody (5H836)
Anti-Phospho-HSF1 (S326) Antibody (5H836)
还可以产品编号 TMAH-00909 别名 Phospho-HSF1 (S326), HSTF1, heat shock transcription factor 1
Anti-Phospho-HSF1 (Ser362) Antibody (5H836) 是一种抗体，靶向 Phospho-HSF1 (Ser362)。Anti-Phospho-HSF1 (Ser362) Antibody (5H836) 可用于 ELISA, WB, ICC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-Phospho-HSF1 (Ser362) Antibody (5H836) is an antibody targeting Phospho-HSF1 (Ser362). Anti-Phospho-HSF1 (Ser362) Antibody (5H836) can be used in ELISA, WB, ICC. |
| 别名 | Phospho-HSF1 (S326), HSTF1, heat shock transcription factor 1 |
| Ig Type | Rabbit IgG |
| 克隆号 | 5H836 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in Hela whole cell lysate -All lanes Phospho-HSF1 antibody at 0.8μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 82 KDa -Observed band size: 82 KDa 2. Immunocytochemistry analysis of TMAH-00909 diluted at 1:80 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| 应用 | ELISA, WB, ICC |
| 推荐剂量 | WB:1:500-1:5000; ICC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Cytoplasm. Nucleus, nucleoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site... |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Phospho-HSF1 (S326) |
| 抗原种属 | Human |
| 基因ID | 3297 |
| Uniprot ID | |
| 研究领域 | Tags & Cell Markers |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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