Anti-Phospho-Histone H3 (Ser28) Antibody (4M267) is a Mouse antibody targeting Phospho-Histone H3 (Ser28). Anti-Phospho-Histone H3 (Ser28) Antibody (4M267) can be used in ELISA, FC.

IgG1

4M267

Human

1. Overlay Peak curve showing Hela cells stained with TMAH-00905 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
2. Overlay Peak curve showing HepG2 cells stained with TMAH-00905 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, FC

Monoclonal

Unconjugated

KS(Phospho)APATGGVKKPHRC

Human

8350

Epigenetics and Nuclear Signaling