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Anti-Phospho-Histone H3 (Ser28) Antibody (3I430)
还可以产品编号 TMAY-01718
Anti-Phospho-Histone H3 (Ser28) Antibody (3I430) 是一种 Rabbit 抗体，靶向 Phospho-Histone H3 (Ser28)。Anti-Phospho-Histone H3 (Ser28) Antibody (3I430) 可用于 WB。
Anti-Phospho-Histone H3 (Ser28) Antibody (3I430)
Anti-Phospho-Histone H3 (Ser28) Antibody (3I430)
还可以产品编号 TMAY-01718
Anti-Phospho-Histone H3 (Ser28) Antibody (3I430) 是一种 Rabbit 抗体，靶向 Phospho-Histone H3 (Ser28)。Anti-Phospho-Histone H3 (Ser28) Antibody (3I430) 可用于 WB。
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抗原信息
| 产品描述 | Anti-Phospho-Histone H3 (Ser28) Antibody (3I430) is a Rabbit antibody targeting Phospho-Histone H3 (Ser28). Anti-Phospho-Histone H3 (Ser28) Antibody (3I430) can be used in WB. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 3I430 |
| 交叉反应 | Human; Predicted to React with:Species predicted to react based on 100% sequence homology: Mouse, Rat |
| 验证活性 | 1. Western blot analysis of extracts from serum-starved Hela, untreated (line A) or treated with calyculin A(200nM, 30min; +)(line B), using Phospho-Histone H3 (Ser28) rabbit monoclonal Antibody at 1:20000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution (lower). 2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with calyculin A(200nM, 30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Histone H3 (Ser28) rabbit monoclonal Antibody at 1:20000 dilution. (Validation Experiment) |
| 应用 | WB |
| 推荐剂量 | WB: 1:50000-1:500000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around (Ser28) of Human Histone H3. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the residues around (Ser28) of Human Histone H3 |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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