Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GCN2 (Thr899). Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody can be used in FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Cow,Horse)

1. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Phospho-GCN2 (Thr899) antibody (TMAB-01415)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-Phospho-GCN2 (Thr899) antibody (TMAB-01415) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 1ug/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human GCN2 around the phosphorylation site of Thr899

Human

EIF2AK4

eIF-2-alpha kinase GCN2

Regulation,Other Kinases

Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.