Anti-Phospho-eIF2α (Ser51) Antibody (9Z756) is a Rabbit antibody targeting Phospho-eIF2α (Ser51). Anti-Phospho-eIF2α (Ser51) Antibody (9Z756) can be used in WB.

Monoclonal Rabbit IgG

9Z756

Human;

Species predicted to react based on 100% sequence homology: Mouse, rat

1. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-eIF2α (Ser51) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment)

2. Western blot analysis of extracts from serum-starved Hela, treated with Calyculin A (100 nM, 30 min), using Phospho-eIF2α (Ser51) Antibody and other brands’ antibodies (company C) at dilution of 1:1000, 1:10000 and 1:200000. (Validation Experiment)

3. Western blot analysis of extracts from serum-starved NIH-3T3, untreated(line A) or treated with CalyculinA( 100nM, 30min; +)(line B), using Phospho-eIF2α (Ser51) rabbit monoclonal Antibody at 1:1000 dilution.

WB

WB: 1:1000-1:10000

Monoclonal

Unconjugated

A synthetic phosphopeptide corresponding to residues around Ser51 of human Phospho-eIF2α.

Human