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顶部生物活性储存和溶解信息计算器

Anti-Phospho-eIF2α (Ser51) Antibody (1F908)

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产品编号 TMAC-03210

别名 Phospho-eIF2α (S51), p-eIF2α (Ser51), p-eIF2α (S51), eIF2α (p-Ser51), eIF2α (p-S51), EIF-2α, EIF-2alpha, EIF2alpha, EIF-2A, EIF2A, EIF-2, EIF2

Anti-Phospho-eIF2α (Ser51) Antibody (1F908) 是一种 Rabbit 抗体，靶向 Phospho-eIF2α (Ser51)。Anti-Phospho-eIF2α (Ser51) Antibody (1F908) 可用于 WB,ICC/IF,IHC,IP,FCM。

Anti-Phospho-eIF2α (Ser51) Antibody (1F908)

Anti-Phospho-eIF2α (Ser51) Antibody (1F908)

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产品编号 TMAC-03210 别名 Phospho-eIF2α (S51), p-eIF2α (Ser51), p-eIF2α (S51), eIF2α (p-Ser51), eIF2α (p-S51), EIF-2α, EIF-2alpha, EIF2alpha, EIF-2A, EIF2A, EIF-2, EIF2

Anti-Phospho-eIF2α (Ser51) Antibody (1F908) 是一种 Rabbit 抗体，靶向 Phospho-eIF2α (Ser51)。Anti-Phospho-eIF2α (Ser51) Antibody (1F908) 可用于 WB,ICC/IF,IHC,IP,FCM。

规格价格库存数量
50 μL
¥ 1,490
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100 μL
¥ 2,495
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产品介绍

生物活性
产品描述
Anti-Phospho-eIF2α (Ser51) Antibody (1F908) is a Rabbit antibody targeting Phospho-eIF2α (Ser51). Anti-Phospho-eIF2α (Ser51) Antibody (1F908) can be used in FCM,ICC/IF,IHC,IP,WB.
别名Phospho-eIF2α (S51), p-eIF2α (Ser51), p-eIF2α (S51), eIF2α (p-Ser51), eIF2α (p-S51), EIF-2α, EIF-2alpha, EIF2alpha, EIF-2A, EIF2A, EIF-2, EIF2
Ig Type
IgG
克隆号
1F908
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Phospho-eIF-2a (S51) on different lysates using anti-Phospho-eIF-2a (S51) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: HUVEC, Lane 3: PC12.
2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.
7. ICC staining Phospho-eIF-2a (S51) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Hela cells with Phospho-eIF-2a (S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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应用FCMICC/IFIHCIPWB
推荐剂量
WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
A synthesized phosphopeptide: human eIF-2a around the phosphorylation site of Ser51
抗原种属
human
Uniprot ID
Q9BY44
化学信息
分子量Theoretical: 36 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

  • 稀释 计算器

关键词

peIF2α (Ser51)peIF2α (S51)eIF2α (pSer51)eIF2α (pS51)eIF2α (phospho-Ser51)eIF2α (phospho-S51)

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