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Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody

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Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody
产品编号 TMAB-01399

Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-CaMKII (Thr287)。Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。

Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody

Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody

Rating icon 还可以
Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody
产品编号 TMAB-01399

Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-CaMKII (Thr287)。Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CaMKII (Thr287). Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Rat (predicted:Mouse,Chicken,Rabbit,Sheep)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
应用
推荐剂量
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell junction, synapse, presynaptic cell membrane. Cell junction, synapse. Note=Postsynaptic lipid rafts.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008].
抗原信息
免疫原
KLH conjugated Synthesised phosphopeptide: human CaMK2 alpha around the phosphorylation site of Thr287
抗原种属
Human
基因ID
Uniprot ID
研究领域
Calmodulin / CaMK,Hypoxia,Hypertrophy,Response to hypoxia
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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