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Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-CaMKII (Thr287)。Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。
Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-CaMKII (Thr287)。Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,160 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CaMKII (Thr287). Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Rat (predicted:Mouse,Chicken,Rabbit,Sheep) |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
应用 | |
推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cell junction, synapse, presynaptic cell membrane. Cell junction, synapse. Note=Postsynaptic lipid rafts. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]. |
免疫原 | KLH conjugated Synthesised phosphopeptide: human CaMK2 alpha around the phosphorylation site of Thr287 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Calmodulin / CaMK,Hypoxia,Hypertrophy,Response to hypoxia |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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