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顶部生物活性储存和溶解信息计算器

Anti-Phospho-AKT1 (Ser473) Antibody (6A70)

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产品编号 TMAC-03188

别名 p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473)

Anti-Phospho-AKT1 (Ser473) Antibody (6A70) 是一种 Rabbit 抗体，靶向 Phospho-AKT1 (Ser473)。Anti-Phospho-AKT1 (Ser473) Antibody (6A70) 可用于 ICC/IF,IHC,WB。

Anti-Phospho-AKT1 (Ser473) Antibody (6A70)

Anti-Phospho-AKT1 (Ser473) Antibody (6A70)

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Rating icon 还可以
产品编号 TMAC-03188 别名 p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473)

Anti-Phospho-AKT1 (Ser473) Antibody (6A70) 是一种 Rabbit 抗体，靶向 Phospho-AKT1 (Ser473)。Anti-Phospho-AKT1 (Ser473) Antibody (6A70) 可用于 ICC/IF,IHC,WB。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-AKT1 (Ser473) Antibody (6A70) is a Rabbit antibody targeting Phospho-AKT1 (Ser473). Anti-Phospho-AKT1 (Ser473) Antibody (6A70) can be used in ICC/IF,IHC,WB.
别名p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473)
Ig Type
IgG
克隆号
6A70
交叉反应
Human,Mouse
验证活性
1. Western blot analysis of Phospho-Akt1(Ser473) on different lysates using anti-Phospho-Akt1 (Ser473) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 treated with PDGF, Lane 2: NIH/3T3 untreated.
2. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Phospho-Akt1 (Ser473) antibody. Counter stained with hematoxylin.
3. ICC staining Phospho-Akt1 (Ser473) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivity
应用ICC/IFIHCWB
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Hybridoma Monoclonal Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
A synthesized phosphopeptide: human Akt1 around the phosphorylation site of Ser473
抗原种属
human
Uniprot ID
P31749
化学信息
分子量Theoretical: 56 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

pAKT1 (Ser473)pAKT1 (S473)AKT1 (pSer473)AKT1 (pS473)AKT1 (phospho-Ser473)AKT1 (phospho-S473)

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4个月前
5.0
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