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Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody
还可以产品编号 TMAC-00115
别名 p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473)
Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-AKT1 (Ser473)。Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody 可用于 IF,IHC,WB。
Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody
Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody
一键复制产品信息 还可以产品编号 TMAC-00115 别名 p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473)
Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-AKT1 (Ser473)。Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody 可用于 IF,IHC,WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,235 | 5日内发货 |
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Ser473). Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody can be used in IF,IHC,WB. |
| 别名 | p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473) |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt(Phospho-Ser473) Antibody TMAC-00115(left) or the same antibody preincubated with blocking peptide(right). 2. Western blot analysis of extracts from SK-BR-3 cells,treated with insulin and EGF, and pretreated with U0126and LY294002 cells using Akt (Phospho-Ser473) AntibodyTMAC-00115. 3. Immunohistochemical analysis of paraffinembeddedhuman Lung carcinoma tissue usingAkt (Phospho-Ser473) Antibody TMAC-00115. 4. Western blot analysis of extracts of various cell lines, using Akt(Phospho-Ser473) TMAC-00115. 5. Western blot analysis of various lysates using Akt(Phospho-Ser473) Antibody at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (L3012) at 1:10000 dilution. Lysates/proteins: 25μg per lane.      |
| 应用 | IFIHCWB |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 性状 | Liquid |
| 缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | Phospho |
| 免疫原 | Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from Human Akt |
| 抗原种属 | human |
| Uniprot ID |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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