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Anti-Phospho-AKT (Ser473) Antibody (8S643)
还可以产品编号 TMAY-01580
Anti-Phospho-AKT (Ser473) Antibody (8S643) 是一种 Rabbit 抗体，靶向 Phospho-AKT (Ser473)。Anti-Phospho-AKT (Ser473) Antibody (8S643) 可用于 WB,IHC-P。
Anti-Phospho-AKT (Ser473) Antibody (8S643)
Anti-Phospho-AKT (Ser473) Antibody (8S643)
还可以产品编号 TMAY-01580
Anti-Phospho-AKT (Ser473) Antibody (8S643) 是一种 Rabbit 抗体，靶向 Phospho-AKT (Ser473)。Anti-Phospho-AKT (Ser473) Antibody (8S643) 可用于 WB,IHC-P。
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抗原信息
| 产品描述 | Anti-Phospho-AKT (Ser473) Antibody (8S643) is a Rabbit antibody targeting Phospho-AKT (Ser473). Anti-Phospho-AKT (Ser473) Antibody (8S643) can be used in WB,IHC-P. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8S643 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution. (Validation Experiment) 2. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution. 3. Western blot analysis of extracts from serum-starved NIH-3T3 treated with PDGFA (5 μg/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:1000, 1:50000, 1:200000 dilution. (Validation Experiment) 4. Western blot analysis of extracts from serum-starved A431, untreated (-); treated with EGF (200 ng/mL, 15 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower). (Validation Experiment) 5. Western blot analysis of extracts from serum-starved NIH-3T3, untreated (-); treated with PDGFA (5 μg/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower). 6. Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 μg/mL, 5 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution. (Validation Experiment) 7. Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5000 ng/mL, 5min; +) (line B); treated with PDGFA and λ-phosphatase (line C) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution. (Validation Experiment) |
| 应用 | WB,IHC-P |
| 推荐剂量 | WB: 1:1000-1:200000; IHC-P: 1:100-1:400 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | The antibody is produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues around Ser473 of human AKT. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | v-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic phosphopeptide corresponding to residues around Ser473 of human AKT. |
| 抗原种属 | Human |
| 研究领域 | Cancer Drug Targets |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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