Anti-PGC1 alpha/beta Polyclonal Antibody is a Rabbit antibody targeting PGC1 alpha/beta. Anti-PGC1 alpha/beta Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit)

1. Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-PGC1 alpha+beta antibody (TMAB-01375)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
Lane 1: Molt-4 (Human) Cell Lysate at 30 μg
Lane 2: A431 (Human) Cell Lysate at 30 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: Hela (Human) Cell Lysate at 30 μg
Lane 5: Jurkat (Human) Cell Lysate at 30 μg
Lane 6: HL60 (Human) Cell Lysate at 30 μg
Primary: Anti-PGC1 alpha + beta (TMAB-01375) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90/113 kDa
Observed band size: 113 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PGC1 alpha + beta) Polyclonal Antibody, Unconjugated (TMAB-01375) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human PGC1 alpha + beta

Human

PPARGC1A

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha

Diabetes associated,Metabolism,Co-activators/co-repressors,Estrogen,Other factors,Energy Metabolism,Fatty acids,Mitochondrial markers,Fatty acid oxidation,Diabetes,Heart disease,Obesity,Energy Metabolism,Mitochondrial

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism.