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Anti-PDCD6IP Antibody (2I749)
还可以产品编号 TMAH-00866
别名 Programmed cell death 6-interacting protein, PDCD6-interacting protein, KIAA1375, Hp95, ALIX, ALG-2-interacting protein X, ALG-2-interacting protein 1, AIP1
Anti-PDCD6IP Antibody (2I749) 是一种抗体，靶向 PDCD6IP。Anti-PDCD6IP Antibody (2I749) 可用于 ELISA, WB, FC。
Anti-PDCD6IP Antibody (2I749)
Anti-PDCD6IP Antibody (2I749)
还可以产品编号 TMAH-00866 别名 Programmed cell death 6-interacting protein, PDCD6-interacting protein, KIAA1375, Hp95, ALIX, ALG-2-interacting protein X, ALG-2-interacting protein 1, AIP1
Anti-PDCD6IP Antibody (2I749) 是一种抗体，靶向 PDCD6IP。Anti-PDCD6IP Antibody (2I749) 可用于 ELISA, WB, FC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-PDCD6IP Antibody (2I749) is an antibody targeting PDCD6IP. Anti-PDCD6IP Antibody (2I749) can be used in ELISA, WB, FC. |
| 别名 | Programmed cell death 6-interacting protein, PDCD6-interacting protein, KIAA1375, Hp95, ALIX, ALG-2-interacting protein X, ALG-2-interacting protein 1, AIP1 |
| Ig Type | Rabbit IgG |
| 克隆号 | 2I749 |
| 交叉反应 | Human, Rat |
| 验证活性 | 1. Western Blot -Positive WB detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate -All lanes: ALIX antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 97, 97, 31 kDa -Observed band size: 97 kDa 2. Overlay histogram showing Jurkat cells stained with TMAH-00866 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISA, WB, FC |
| 推荐剂量 | WB:1:500-1:5000; FC:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm, cytosol. Melanosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Secreted, extracellular exosome. Cell junction, tight junction. Midbody, Midbody ring. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP. By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier. (Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses). |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human ALIX |
| 抗原种属 | Human |
| 基因ID | 10015 |
| Uniprot ID | |
| 研究领域 | Cell biology, Microbiology, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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