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Anti-PCNA Antibody (8X645) 是一种 Mouse 抗体,靶向 PCNA。Anti-PCNA Antibody (8X645) 可用于 WB,IHC,FCM。
Anti-PCNA Antibody (8X645) 是一种 Mouse 抗体,靶向 PCNA。Anti-PCNA Antibody (8X645) 可用于 WB,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,990 | 5日内发货 | |
100 μL | ¥ 2,990 | 5日内发货 |
产品描述 | Anti-PCNA Antibody (8X645) is a Mouse antibody targeting PCNA. Anti-PCNA Antibody (8X645) can be used in WB,IHC,FCM. |
别名 | proliferating cell nuclear antigen, ATLD2 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of PCNA on different cell lysates using anti-PCNA antibody at 1/1000 dilution. Positive control: Line 1: L929, Line 2: MCF-7, Line 3: PC12, Line 4: Raji, Line 5: F9, Line 6: A549. 2. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PCNA antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-PCNA antibody. Counter stained with hematoxylin. 6. Flow cytometric analysis of Hela cells with PCNA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:200; FCM: 1:100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | PCNA (Proliferating cell nuclear antigen) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. It induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 29 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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