Anti-PCDH20 Polyclonal Antibody is a Rabbit antibody targeting PCDH20. Anti-PCDH20 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Horse,Rabbit)

1. Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-PCDH20 antibody (TMAB-10049), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
2. Sample:
Hippocampus (Mouse) Lysate at 40 μg
Olfactory bulb (Mouse) Lysate at 40 μg
Primary: Anti-PCDH20 (TMAB-10049) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 100 kD
3. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SV2B) Polyclonal Antibody, Unconjugated (TMAB-10049) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human U87MG cell lysates
Primary: Anti-PCDH20 (TMAB-10049) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 102 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human PCDH20

Human

PCDH20

Protocadherin-20

Potential calcium-dependent cell-adhesion protein.