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顶部生物活性储存和溶解信息计算器

Anti-PAX5 Antibody (2B642)

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产品编号 TMAC-03076

别名 PAX5, Paired box protein Pax-5, B-cell-specific transcription factor (BSAP)

Anti-PAX5 Antibody (2B642) 是一种 Rabbit 抗体，靶向 PAX5。Anti-PAX5 Antibody (2B642) 可用于 FCM,ICC/IF,IHC,WB。

Anti-PAX5 Antibody (2B642)

Anti-PAX5 Antibody (2B642)

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Rating icon 还可以
产品编号 TMAC-03076 别名 PAX5, Paired box protein Pax-5, B-cell-specific transcription factor (BSAP)

Anti-PAX5 Antibody (2B642) 是一种 Rabbit 抗体，靶向 PAX5。Anti-PAX5 Antibody (2B642) 可用于 FCM,ICC/IF,IHC,WB。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-PAX5 Antibody (2B642) is a Rabbit antibody targeting PAX5. Anti-PAX5 Antibody (2B642) can be used in FCM,ICC/IF,IHC,WB.
别名PAX5, Paired box protein Pax-5, B-cell-specific transcription factor (BSAP)
Ig Type
IgG
克隆号
2B642
交叉反应
Human,Mouse
验证活性
1. Western blot analysis of PAX5 on Daudi cells lysates using anti-PAX5 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PAX5 antibody. Counter stained with hematoxylin.
3. ICC staining PAX5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining PAX5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining PAX5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of Raji cells with PAX5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用FCMICC/IFIHCWB
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The Pax family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. Pax family genes are also involved in a variety of signal transduction pathways in the adult organism. Mutations in Pax proteins have been linked to disease and cancer in humans. For example, the human PAX5 gene encodes a B cell lineage-specific protein, Pax-5, also designated B cell specific activator protein or BSAP, which is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. Pax-5 functions to regulate not only B cell development, but also influences the balance between immunoglobulin secretion and B cell proliferation. Overexpression of Pax-5 has been implicated in cellular transformation, and in the case of small lymphocytic lymphomas with plasmacytoid differentiation, a t(9;14)(p13;q32) translocation resulting in the deregulation of PAX5 gene expression has been detected.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q02548
化学信息
分子量Theoretical: 42 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

PAX 5BSAP

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