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Anti-PARP1 Antibody (4Z18)
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产品编号 TMAC-03070
别名 PPOL, Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT
Anti-PARP1 Antibody (4Z18) 是一种 Rabbit 抗体，靶向 PARP1。Anti-PARP1 Antibody (4Z18) 可用于 WB,ICC/IF,IHC,FCM。
Anti-PARP1 Antibody (4Z18)
Anti-PARP1 Antibody (4Z18)
还可以产品编号 TMAC-03070 别名 PPOL, Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT
Anti-PARP1 Antibody (4Z18) 是一种 Rabbit 抗体，靶向 PARP1。Anti-PARP1 Antibody (4Z18) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PARP1 Antibody (4Z18) is a Rabbit antibody targeting PARP1. Anti-PARP1 Antibody (4Z18) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | PPOL, Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PARP on different lysates using anti-PARP antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: Jurkat, Lane 3: Hela. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PARP antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PARP antibody. Counter stained with hematoxylin. 4. ICC staining PARP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 113/89 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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