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顶部生物活性储存和溶解信息计算器

Anti-PARP1 Antibody (4Z18)

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产品编号 TMAC-03070

别名 PPOL, Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT

Anti-PARP1 Antibody (4Z18) 是一种 Rabbit 抗体，靶向 PARP1。Anti-PARP1 Antibody (4Z18) 可用于 FCM,ICC/IF,IHC,WB。

Anti-PARP1 Antibody (4Z18)

Anti-PARP1 Antibody (4Z18)

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Rating icon 还可以
产品编号 TMAC-03070 别名 PPOL, Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT

Anti-PARP1 Antibody (4Z18) 是一种 Rabbit 抗体，靶向 PARP1。Anti-PARP1 Antibody (4Z18) 可用于 FCM,ICC/IF,IHC,WB。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-PARP1 Antibody (4Z18) is a Rabbit antibody targeting PARP1. Anti-PARP1 Antibody (4Z18) can be used in FCM,ICC/IF,IHC,WB.
别名PPOL, Poly-PARP, PARS, PARP-1, PARP, pADPRT-1, ARTD1, ADPRT1, ADPRT 1, ADPRT
Ig Type
IgG
克隆号
4Z18
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of PARP on different lysates using anti-PARP antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: Jurkat, Lane 3: Hela.
2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PARP antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PARP antibody. Counter stained with hematoxylin.
4. ICC staining PARP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. Flow cytometric analysis of Hela cells with PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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应用FCMICC/IFIHCWB
推荐剂量
WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P09874
化学信息
分子量Theoretical: 113/89 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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