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Anti-PAK2 Antibody (7G572)
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产品编号 TMAC-03056
别名 Serine/threonine-protein kinase PAK 2, S6/H4 kinase, PAK65, PAK-2, p58, p21-activated kinase 2, Gamma-PAK, EC 2.7.11.1
Anti-PAK2 Antibody (7G572) 是一种 Rabbit 抗体，靶向 PAK2。Anti-PAK2 Antibody (7G572) 可用于 WB,ICC,IF,IHC,FCM。
Anti-PAK2 Antibody (7G572)
Anti-PAK2 Antibody (7G572)
还可以产品编号 TMAC-03056 别名 Serine/threonine-protein kinase PAK 2, S6/H4 kinase, PAK65, PAK-2, p58, p21-activated kinase 2, Gamma-PAK, EC 2.7.11.1
Anti-PAK2 Antibody (7G572) 是一种 Rabbit 抗体，靶向 PAK2。Anti-PAK2 Antibody (7G572) 可用于 WB,ICC,IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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| 产品描述 | Anti-PAK2 Antibody (7G572) is a Rabbit antibody targeting PAK2. Anti-PAK2 Antibody (7G572) can be used in WB,ICC,IF,IHC,FCM. |
| 别名 | Serine/threonine-protein kinase PAK 2, S6/H4 kinase, PAK65, PAK-2, p58, p21-activated kinase 2, Gamma-PAK, EC 2.7.11.1 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PAK2 on mouse thymus tissue (1) and MCF-7 cell (2) lysate using anti-PAK2 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PAK2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PAK2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PAK2 antibody. Counter stained with hematoxylin. 5. ICC staining PAK2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining PAK2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of A431 cells with PAK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:100-500; ICC: 1:100-500; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Three recently identified isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase STE20, involved in pheromone signaling. The α, β, and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 58 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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