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Anti-PABPN1 Antibody (5O355)
还可以产品编号 TMAH-00848
别名 Polyadenylate-binding protein 2, Polyadenylate-binding nuclear protein 1, Poly(A)-binding protein II, Poly(A)-binding protein 2, PABPN 1, PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1
Anti-PABPN1 Antibody (5O355) 是一种抗体，靶向 PABPN1。Anti-PABPN1 Antibody (5O355) 可用于 ELISA, WB, IHC, IF, FC。
Anti-PABPN1 Antibody (5O355)
Anti-PABPN1 Antibody (5O355)
还可以产品编号 TMAH-00848 别名 Polyadenylate-binding protein 2, Polyadenylate-binding nuclear protein 1, Poly(A)-binding protein II, Poly(A)-binding protein 2, PABPN 1, PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1
Anti-PABPN1 Antibody (5O355) 是一种抗体，靶向 PABPN1。Anti-PABPN1 Antibody (5O355) 可用于 ELISA, WB, IHC, IF, FC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-PABPN1 Antibody (5O355) is an antibody targeting PABPN1. Anti-PABPN1 Antibody (5O355) can be used in ELISA, WB, IHC, IF, FC. |
| 别名 | Polyadenylate-binding protein 2, Polyadenylate-binding nuclear protein 1, Poly(A)-binding protein II, Poly(A)-binding protein 2, PABPN 1, PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1 |
| Ig Type | Rabbit IgG |
| 克隆号 | 5O355 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: 293 whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate -All lanes: PABPN1 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 33, 32, 38 kDa -Observed band size: 50 kDa 2. IHC image of TMAH-00848 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00848 diluted at 1:100 and staining in paraffin-embedded human bladder cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 4. Immunofluorescence staining of Hela Cells with TMAH-00848 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). 5. Overlay histogram showing Hela cells stained with TMAH-00848 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISA, WB, IHC, IF, FC |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200; FC:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Cytoplasm. Nucleus speckle. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human PABPN1 |
| 抗原种属 | Human |
| 基因ID | 8106 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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