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Anti-PABPN1 Antibody (3P321)
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产品编号 TMAC-03046
别名 Polyadenylate-binding protein 2, Polyadenylate-binding nuclear protein 1, Poly(A)-binding protein II, Poly(A)-binding protein 2, PABPN 1, PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1
Anti-PABPN1 Antibody (3P321) 是一种 Rabbit 抗体，靶向 PABPN1。Anti-PABPN1 Antibody (3P321) 可用于 WB,ICC/IF,IHC,FCM,IP。
Anti-PABPN1 Antibody (3P321)
Anti-PABPN1 Antibody (3P321)
还可以产品编号 TMAC-03046 别名 Polyadenylate-binding protein 2, Polyadenylate-binding nuclear protein 1, Poly(A)-binding protein II, Poly(A)-binding protein 2, PABPN 1, PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1
Anti-PABPN1 Antibody (3P321) 是一种 Rabbit 抗体，靶向 PABPN1。Anti-PABPN1 Antibody (3P321) 可用于 WB,ICC/IF,IHC,FCM,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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| 产品描述 | Anti-PABPN1 Antibody (3P321) is a Rabbit antibody targeting PABPN1. Anti-PABPN1 Antibody (3P321) can be used in WB,ICC/IF,IHC,FCM,IP. |
| 别名 | Polyadenylate-binding protein 2, Polyadenylate-binding nuclear protein 1, Poly(A)-binding protein II, Poly(A)-binding protein 2, PABPN 1, PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Western blot analysis of PABPN1 on MCF-7 cells lysates using anti-PABPN1 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PABPN1 antibody. Counter stained with hematoxylin. 3. ICC staining PABPN1 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining PABPN1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with PABPN1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 49 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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