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Anti-p60 CAF1 Antibody (4X99)

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Anti-p60 CAF1 Antibody (4X99)
产品编号 TMAC-03007
别名 p60 subunit, p60 CAF 1, MPP7, MPP 7, MPHOSPH7, M-phase phosphoprotein 7, M phase phosphoprotein 7, Human chromatin assembly factor-I p60 subunit, Chromatin assembly factor I p60 subunit, Chromatin assembly factor I, Chromatin assembly factor 1 subunit B, CHAF1B, CHAF 1B, CAF-I p60, CAF-I 60 kDa subunit, CAF1P60, CAF1B_HUMAN, CAF1A, CAF-1 subunit B, CAF-1, CAF1, CAF IP60, CAF I 60 kDa subunit, CAF 1A, CAF 1 subunit B

Anti-p60 CAF1 Antibody (4X99) 是一种 Rabbit 抗体，靶向 p60 CAF1。Anti-p60 CAF1 Antibody (4X99) 可用于 WB,ICC,IF,IP,IHC,FCM。

Anti-p60 CAF1 Antibody (4X99)

Anti-p60 CAF1 Antibody (4X99)

Rating icon 还可以
Anti-p60 CAF1 Antibody (4X99)
产品编号 TMAC-03007 别名 p60 subunit, p60 CAF 1, MPP7, MPP 7, MPHOSPH7, M-phase phosphoprotein 7, M phase phosphoprotein 7, Human chromatin assembly factor-I p60 subunit, Chromatin assembly factor I p60 subunit, Chromatin assembly factor I, Chromatin assembly factor 1 subunit B, CHAF1B, CHAF 1B, CAF-I p60, CAF-I 60 kDa subunit, CAF1P60, CAF1B_HUMAN, CAF1A, CAF-1 subunit B, CAF-1, CAF1, CAF IP60, CAF I 60 kDa subunit, CAF 1A, CAF 1 subunit B

Anti-p60 CAF1 Antibody (4X99) 是一种 Rabbit 抗体，靶向 p60 CAF1。Anti-p60 CAF1 Antibody (4X99) 可用于 WB,ICC,IF,IP,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-p60 CAF1 Antibody (4X99) is a Rabbit antibody targeting p60 CAF1. Anti-p60 CAF1 Antibody (4X99) can be used in WB,ICC,IF,IP,IHC,FCM.
别名p60 subunit, p60 CAF 1, MPP7, MPP 7, MPHOSPH7, M-phase phosphoprotein 7, M phase phosphoprotein 7, Human chromatin assembly factor-I p60 subunit, Chromatin assembly factor I p60 subunit, Chromatin assembly factor I, Chromatin assembly factor 1 subunit B, CHAF1B, CHAF 1B, CAF-I p60, CAF-I 60 kDa subunit, CAF1P60, CAF1B_HUMAN, CAF1A, CAF-1 subunit B, CAF-1, CAF1, CAF IP60, CAF I 60 kDa subunit, CAF 1A, CAF 1 subunit B
Ig Type
IgG
交叉反应
Human
验证活性
1. Western blot analysis of p60 CAF1 on different cell lysates using anti-p60 CAF1 antibody at 1/1,000 dilution. Positive control: Lane 1: SiHa, Lane 2: K562, Lane 3: A431.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p60 CAF1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p60 CAF1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-p60 CAF1 antibody. Counter stained with hematoxylin.
5. ICC staining p60 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining p60 CAF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining p60 CAF1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of K562 cells with p60 CAF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
应用
WB
ICC
IF
IP
IHC
FCM
推荐剂量
WB: 1:500-2000; IHC: 1:50-200; ICC:IF: 1:50-200; IP: 1:10-50; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: the C-terminus of Human p60 CAF1
抗原种属
human
Uniprot ID
Q13112
化学信息
分子量Theoretical: 61 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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